Characterization of potential probiotics Lactobacillus species isolated from the gastrointestinal tract of Rhode Island Red (RIR) chicken in Ethiopia

Background and aimsNowadays, probiotic microorganisms are given high attention due to their potential for the improvement of animal production productivity. The natural gut microflora poultry can serve as an excellent source optimum strains. Therefore, this study isolated, identified characterized Lactobacillus strains from digestive tract content RIR chicken in Ethiopia.MethodsA total five chickens were randomly taken a sample was each gastrointestinal further analysis. For characterization, among 190 isolates only 10 representative vitro characterization. selected screened based on biochemical, morphological, 16S rRNA gene sequences.ResultsSurvival IS3, IS4, IS6, IS7 significantly different (P < 0.05) at pH 2. showed tolerance 0.3% bile salt. Isolates IS1, IS2, IS5, IS7, IS8 ampicillin-resistant, chloramphenicol, ciprofloxacin, erythromycin used antibiotics. All ten antagonistic activity against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, intermedius, enteritidis. temperature all 45 °C, could grow 0.69 mol/L NaCl. Using phylogenetic analysis 16SrRNA sequence; IS3 salivarius, while reuteri.ConclusionsThe results indicated that survive stress conditions thus be considered candidates chickens.